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當(dāng)前位置: 首頁(yè) > ATCC代理 > pHUK-8 [pHUK-1] B228600
pHUK-8 [pHUK-1]
pHUK-8 [pHUK-1]
規(guī)格:
價(jià)格:
編號(hào):B228600
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pHUK-8 [pHUK-1]
商品貨號(hào)
B228600
Designations
pHUK-8 [pHUK-1]
GenBank Number

K02286

培養(yǎng)基
Species
Homo sapiens, human
Depositors
F Blasi
Applications
Enzyme(s) not detecting polymorphism: EcoRI, HindIII, PstI, PvuII, SmaI, SstI, MspI, TaqI. Tested against 8 unrelated individuals.
Vector
Construct size (kb): 5.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.5
Tissue: SV40-transformed fibroblast
Gene product: plasminogen activator, urokinase [PLAU]
Alleles: A1, A2
Insert Size (kb)
1.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.9, 1.5; BamHI--4.3, 1.2; AvaI--5.6; HindIII--5.8; EcoRI--4.6, 0.63, 0.42.
Includes unspliced intervening sequences and 3' non-coding sequences. Encodes from about aa 103 through 270 bp 3' untranslated. This is a subclone of pHUK-1.
BamHI RFLP has demonstrated codominant segregation in an informative family of 7.
Enzyme(s) not detecting polymorphism: EcoRI, HindIII, PstI, PvuII, SmaI, SstI, MspI, TaqI. Tested against 8 unrelated individuals.
References

Verde P, et al. Identification and primary sequence of an unspliced human urokinase poly(A)+ RNA. Proc. Natl. Acad. Sci. USA 81: 4727-4731, 1984. PubMed: 6589620

Sebastio G, et al. BamHI RFLP linked to the human urokinase gene. Nucleic Acids Res. 13: 5404, 1985. PubMed: 2991863

Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050

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