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pTAM2.5-a

規格:

價格:

編號:B228692

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pTAM2.5-a

商品貨號

B228692

Designations

pTAM2.5-a

GenBank Number

J03520

培養基

Species

Mus musculus, mouse

Depositors

S Strickland

Vector

Construct size (kb): 5.480000019073486

Insert

DNA: cDNA

Insert lengths(kb): 2.519000053405762

Tissue: teratocarcinoma F9 cell line + parietal endoderm

Gene product: plasminogen activator, tissue [Plat]

Insert Size (kb)

2.519

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.7, 0.8; BamHI--5.5; XbaI--4.3, 1.25; HindIII--5.5; XhoI--5.5; BamHI+HindIII--3.0, 2.5.

Transcription from the vector T7 promoter yields sense transcripts.

Full-length cDNA clone which includes 87 bp of 5' non-coding sequence and 745 bp of 3' non-coding sequence.

This clone was constructed by Bill Richards from the 5' end of clambdaEPE (parietal endoderm) and the EcoRI site to the 3' end of clambda2 (F9 teratocarcinoma).

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Rickles RJ, et al. Molecular cloning of complementary DNA to mouse tissue plasminogen activator mRNA and its expression during F9 teratocarcinoma cell differentiation. J. Biol. Chem. 263: 1563-1569, 1988. PubMed: 2826484

Sidney Strickland, personal communication

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