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pPGBC9

規格:

價格:

編號:B228869

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pPGBC9

商品貨號

B228869

Designations

pPGBC9

Species

Homo sapiens, human

培養基

Depositors

P van den Elsen

Applications

Codominant segregation was observed in 4 unrelated families (49 individuals).

Plasmid pPGBC9 was cut with BamHI and the fragments of 0.5 kb and 0.6 kb were isolated, pooled and used as the probe for the MspI and TaqI RFLPs.

Vector

Construct size (kb): 3.900000095367432

Insert

DNA: cDNA

Insert lengths(kb): 0.699999988079071

Tissue: T4+,T8- T-cell clone

Gene product: antigen CD3D, delta polypeptide (TiT3 complex) [T3D]

Alleles: B2, B2, B1, B1, , , , , , , , A1, A2, , , , , , , , A1, A2

Insert Size (kb)

0.700

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): HindIII--3.9; SalI--3.9; PstI--2.5, 1.3; BamHI--2.9, 0.6, 0.5.

TaqI and MspI RFLP alleles are in strong linkage disequilibrium.

Codominant segregation was observed in 4 unrelated families (49 individuals).

The insert contains ATG at 97, TGA at 610, and 89 bp of 3' untranslated sequence including AATAA followed by poly(A) 17 bp downstream.

Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.

Plasmid pPGBC9 was cut with BamHI and the fragments of 0.5 kb and 0.6 kb were isolated, pooled and used as the probe for the MspI and TaqI RFLPs.

Enzyme(s) not detecting polymorphism: RsaI, BstEII, EcoRV, BamHI, HindIII, BglII, PvuII, BglI and PstI in at least 6 unrelated individuals.

References

Malhotra U, Concannon P. Human T-cell receptor CD3-delta (CD3D) / MspI DNA polymorphism. Nucleic Acids Res. 17: 2373, 1989. PubMed: 2468134

van den Elsen P, et al. Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex. Nature 312: 413-418, 1984. PubMed: 6095101

Charmley P, et al. A primary linkage map of the human chromosome 11q22-23 region. Genomics 6: 316-323, 1990. PubMed: 2307473

Barbara Weiffenbach, personal communication

B193664：pPGBC9

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