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HuT 78
HuT 78
規格:
價格:
編號:B229028
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HuT 78
商品貨號
B229028
Organism
Homo sapiens, human
Cell Type
cutaneous T lymphocyte (KRAS CRM)
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Sezary Syndrome
Age
53 years adult
Gender
male
Ethnicity
Caucasian
Applications
For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. 

This cell line is suitable as a transfection host.

Intended Use

Certified Reference Material produced under an ISO 17034 accredited process.


    ACLASS Accredited Reference Material Producer AR-1384

Storage Conditions
liquid nitrogen vapor phase
Derivation
Hut 78 was derived from peripheral blood of a patient with Sezary syndrome.
Clinical Data
53 years adult
Caucasian
male
Antigen Expression
CD4; Homo sapiens
Receptor Expression
interleukin 2 (IL-2), expressed
Genes Expressed
interleukin 2, tumor necrosis factor alpha (TNF alpha), CD4; Homo sapiens
Cellular Products
interleukin 2
tumor necrosis factor alpha (TNF alpha)
Tumorigenic
Yes
Effects
Yes, in nude mice when injected intracranially
Comments
Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

Certified Reference Material produced under an ISO 17034 accredited process.

Item has been tested for KRAS mutation; KRAS wildtype

The line has the properties of a mature human T cell with helper/inducer activity. It releases IL-2, and has receptors for IL-2. The growth rate is stimulated by IL-2. TNF alpha is an autocrine growth factor for Hut 78.


Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 X 105 viable cells/mL. Maintain cultures at cell concentrations between 5 X 104 and 8 X 105 viable cells/mL. maintain cell density at less than 1 X 106 cells/mL.
Medium Renewal: Two to three times weekly
Cryopreservation
Freeze Medium: Complete growth medium 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11,12
D5S818: 11,12
D7S820: 8,11
THO1: 8,9
TPOX: 8,9
vWA: 14,15
Population Doubling Time
about 65 hours
Name of Depositor
AF Gazdar
Deposited As
Homo sapiens
References

Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346

Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177

Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013

Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674

O'Connell MA, et al. Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78. J. Biol. Chem. 270: 7399-7404, 1995. PubMed: 7706285

Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991

Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128

Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082

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