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HuBS3-Abeta (NH2) [M29551, SGC31484]

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編號:B229212

品牌:Mingzhoubio

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產品名稱

HuBS3-Abeta (NH2) [M29551, SGC31484]

商品貨號

B229212

Designations

HuBS3-Abeta (NH2) [M29551, SGC31484]

GenBank Number

M29551

培養基

Species

Homo sapiens, human

Vector

Construct size (kb): 44.90000152587891

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt11
Intact vector size: 43.700
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.2 kb
Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5)
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
promoter: lac
replicon: lambda
repressor gene: cI857
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PPP3CB
Other gene symbols: CALNA2
Genomic copy number: unique
Gene name: protein phosphatase 3 (formerly 2B), catalytic subunit,
beta isoform (calcineurin A beta)
Contains complete coding sequence?: U
Chromosome: 10; Localization: 10; 10, 448.339999 cR
Tissue: brainstem
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.215
Cross references: DNA Seq. Acc.: M29551

Insert lengths(kb): 1.2150000333786

Tissue: brainstem

Gene product: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform (calcineurin A beta)(protein phosphatase 3, catalytic subunit, beta isoform) [CALNA2]

Insert Size (kb)

1.215

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--24.2, 19.5, 1.2; ClaI--12.5, 6 (doublet), 4.2, 4.0, 2.7, 2.6, 2.0, 1.85, 1.1 (doublet), 0.95, 0.6, 0.35;

HindIII-->23.0, 9.4, 6.2, 3.8, 0.56; SmaI--19.5, 10.5, 8.3, 5.8; SstI--20.0, 18.5, 4.45, 1.35.

The insert contains the following restriction sites (approximate kb from the 5' end): ClaI--0.97; HindIII--0.67, 0.68; NcoI--0.42; SacI--0.98; SmaI--0.10, 0.14, 0.18.

Corresponds to nt 1-1215 of the GenBank record, including the 5' portion of the coding region (nt 117-1691). This is the sequence for the more prevalent type II isoform, and lacks 54 bp in this region relative to the type I.

References

Guerini D, Klee CB. Cloning of human calcineurin A: evidence for two isozymes and identification of a polyproline structural domain. Proc. Natl. Acad. Sci. USA 86: 9183-9187, 1989. PubMed: 2556704

B181906：HuBS3-Abeta (NH2) [M29551, SGC31484]

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