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pmigf2-3

規格:

價格:

編號:B229289

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pmigf2-3

商品貨號

B229289

Designations

pmigf2-3

GenBank Number

M14951

培養基

Species

Mus musculus, mouse

Depositors

GI Bell

Vector

Construct size (kb): 4.75

Insert

DNA: cDNA

Insert lengths(kb): 1.447999954223633

Tissue: placenta (19 day)

Gene product: insulin-like growth factor 2 [Igf2]

Insert Size (kb)

1.448

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

The insert includes these restriction sites (nucleotide position according to the published sequence): BamHI--45, 1075; SacI--473.

Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 1.5; HindIII--4.6; BamHI--2.7, 1.0, 0.7; PstI--4.6; SacI--4.6.

This contains the complete coding sequence, plus a 5' untranslated region of 31 bp and a 3' untranslated region of 864 bp.

This detects a major placental transcript of 3.85 kb and a minor transcript of 4.8 kb. It did not hybridize to adult liver poly(A)+ RNA.

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Stempien MM, et al. Sequence of a placental cDNA encoding the mouse insulin-like growth factor II precursor. DNA 5: 357-361, 1986. PubMed: 3780370

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