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N2TBX1
N2TBX1
規格:
價格:
編號:B229615
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
N2TBX1
商品貨號
B229615
Designations
N2TBX1
GenBank Number

M18070

培養基
Species
Mus musculus, mouse
Depositors
DA Westaway
Vector
Construct size (kb): 5.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.150000095367432
Tissue: brain
Gene product: prion protein (p27-30)(prion protein) [Prnp]
Alleles: b, a
Insert Size (kb)
2.150
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Insert contains the following sites separated by (bp)(approximate): EcoRI- 440- PstI- 750- KpnI- 910- EcoRI.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.5, 2.2; PstI--4.6, 0.52; KpnI--4.3, 1.23; HindIII--5.7; BamHI--5.7.
There is 99.6% nucleotide homology between Prn-p of NZW mice (short scrapie incubation period) and I/LnJ mice (long scrapie incubation period).
BstEII detects an AC to GT change at codon 189 which results in Val (I/Ln) or Thr (NZW). It also correlates with a T to C change at codon 108 which results in Phe (I/Ln) or Leu (NZW). XbaI, PstI and EcoRI RFLPs lie 3' to exon 2.
All 3 inbred strains known to have long scrapie incubation periods lack the BstEII site at codon 189. Seventeen short and intermediate incubation period mice have this site.
Isolated from an NZW mouse (short scrapie incubation period). Includes most of exon 2 of murine Prn-p gene.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Westaway D, et al. Distinct prion proteins in short and long scrapie incubation period mice. Cell 51: 651-662, 1987. PubMed: 2890436

David A Westaway, personal communication

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