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p8.1.1

規格:

價格:

編號:B229816

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

p8.1.1

商品貨號

B229816

Designations

p8.1.1

Species

Homo sapiens, human

培養基

Vector

Construct size (kb): 4.400000095367432

Insert

DNA: cDNA

Insert lengths(kb): 1.735000014305115

Tissue: placenta (34 weeks)

Gene product: keratin 8 [KRT8]

Insert Size (kb)

1.735

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.7; HindIII--3.3, 1.1; BamHI--4.4; PstI--3.1, 0.7, 0.4; EcoRI/HindIII--2.7, 1.1, 0.6.

There is an error in the reported sequence at nt 642 which is a T not an A. The encoded amino acid (Ala) remains the same. The change explains the HindIII restriction site yielding a 1.1 kb fragment.

The clone contains the complete coding sequence including 33 bp 5' untranslated and 250 bp 3' untranslated regions.

The clone detects a single mRNA species of approximately 1.9 kb in fetal intestine, placenta, pancreas, lung, liver, heart, and kidney with little in adrenal gland or muscle.

References

Yamamoto R, et al. Cloning and sequence of cDNA for human placental cytokeratin 8. Regulation of the mRNA in trophoblastic cells by cAMP. Mol. Endocrinol. 4: 370-374, 1990. PubMed: 1692965

Jerome F Strauss, personal communication

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