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cDNA 210
cDNA 210
規格:
價格:
編號:B229918
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
cDNA 210
商品貨號
B229918
Designations
cDNA 210
GenBank Number

X63546

培養基
Species
Homo sapiens, human
Applications
The 3.55 kb EcoRI fragment from the insert, which includes both coding regions, has been used as a probe.
Vector
Construct size (kb): 9.300000190734863
Insert
DNA: cDNA
Insert lengths(kb): 6.300000190734863
Tissue: DNA from ED1 cell line (tre-transfected NIH 3T3)
Gene product: tre-2 oncogene(tre-2 oncogene) [TRE2*]
Insert Size (kb)
6.300
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 3.1, 2.4, 0.46; PstI--7.6, 1.45, 0.72; HindIII--5.2, 4.5; PvuII--2.6, 2.5, 1.10, 0.82, 0.74 (doublet), 0.49 (doublet); SalI--9.6.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.41, 3.96; PstI--0.67, 2.10; HindIII--1.92.
Contains two complete open reading frames, from nt 112-2472 and from nt 2460-4220 of the insert.
The 3.55 kb EcoRI fragment from the insert, which includes both coding regions, has been used as a probe. It detects mRNA of 8.2 kb in Ewing's sarcoma cells (the source of the transfected DNA) and other neoplasms.
Corresponds to nt 1586 - 7878 of the sequence record.
References

Nakamura T, et al. A novel transcriptional unit of the tre oncogene widely expressed in human cancer cells. Oncogene 7: 733-741, 1992. PubMed: 1565468

Nakamura T, et al. Nucleotide sequence of mouse L19 ribosomal protein cDNA isolated in screening with tre oncogene probes. DNA Cell Biol. 9: 697-703, 1990. PubMed: 1702292

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