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pRIL6RC.6

規格:

價格:

編號:B229933

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pRIL6RC.6

商品貨號

B229933

Designations

pRIL6RC.6

GenBank Number

J05668

培養基

Species

Rattus norvegicus, rat

Depositors

GH Fey

Vector

Construct size (kb): 7.300000190734863

Insert

DNA: cDNA

Insert lengths(kb): 4.590000152587891

Tissue: liver, acute inflammation

Gene product: interleukin 6 receptor [IL6R]

Insert Size (kb)

4.590

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

The insert includes the following restriction sites (in nucleotide positions from the 5' end of the published sequence): BamHI--748, 1083, 1177; PstI--1586, 3524, 3999, 4060; XbaI--2359.

Restriction digests of the clone give the following sizes (kb): BamHI--6.4, 0.8; EcoRI--4.6, 2.7; HindIII--3.2, 2.2, 1.3, 0.7; PstI--3.1, 1.9, 1.5, 0.5; XbaI--5.2, 2.4.

The insert extends from nucleotide 71 to 4661 of the published sequence, and encodes the complete reading frame (109 - 1495), plus 5' and 3' untranslated regions.

This detects a mRNA of about 5.1 kb in normal and acute-phase rat liver.

References

Baumann M, et al. Molecular cloning, characterization and functional expression of the rat liver interleukin 6 receptor. J. Biol. Chem. 265: 19853-19862, 1990. PubMed: 2174054

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