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pAA3H

規(guī)格:

價(jià)格:

編號(hào):B230000

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pAA3H

商品貨號(hào)

B230000

Designations

pAA3H

Depositors

A Ahmed

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 11.5000000000000000

Vector: pAA3H (cosmid)

Promoters: Promoter none

Construction: pBR322, lambdagal+(EcoRI/HindIII, galE'TK-cos R/L)

Marker(s):ampR,galS

Construct size (kb): 11.5

Features: insert detection: galE'TK
marker(s): ampR, galS
promoter: none
replicon: pMB1
terminator: none
enhancer: none

Applications

vector for constructing genomic libraries

vector permitting positive selection for inserts

Comments

The galE'TK genes are carried on a HindIII/HpaI fragment of approximately 3.0 kb. The galE gene is truncated at the HindIII site. Expression of the gal sequences are regulated by a promoter located between the BamHI and HindIII sites in the tet gene.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

Vector for positive galactose-resistance selection of DNA cloned into the HindIII site.

Derived from pAA3.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 30.0°C

References

Ahmed A. Plasmid vectors for positive galactose-resistance selection of cloned DNA in Escherichia coli. Gene 28: 37-43, 1984. PubMed: 6329914

Shipped

freeze-dried

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