pcDV1 (ATCC 37368) and pL1 (ATCC 37369) should be used together to construct a cDNA library.
The SV40 segment contains KpnI and EcoRI sites for the preparation of vector-primer containing a poly(dT) tail at one end and the polyadenylation sequence from the late region of the virus.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Okayama H, Berg P. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell. Biol. 3: 280-289, 1983. PubMed: 6300662
