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pBR322(Pst)/HLA-DR-Beta-C
pBR322(Pst)/HLA-DR-Beta-C
規(guī)格:
價(jià)格:
編號(hào):B230374
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pBR322(Pst)/HLA-DR-Beta-C
商品貨號(hào)
B230374
Designations
pBR322(Pst)/HLA-DR-Beta-C
Species
Homo sapiens, human
培養(yǎng)基
Depositors
Biogen N.V., JF Haley, Biogen N.V.
Vector
Construct size (kb): 6.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: PstI
Vector end: PstI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: HLA-DRB*
Gene name: major histocompatibility complex, class II, DR beta
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 p21.3
Tissue: B-lymphocyte IBW 9 cell line
Type of DNA: cDNA
Insert end: Modification: GC tails
Insert end: Modification: GC tails
Insert size (kb): 1.2
Cross references:
Insert lengths(kb): 1.200000047683716
Tissue: B lymphocyte, IBW 9 cell line
Gene product: major histocompatibility complex, class II, DR beta 1(HLA class II histocompatibility antigen, DR-1 beta chain) [HLA-DRB*]
Insert Size (kb)
1.200
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.2; HindIII--5.2, 1.2; PstI--5.8, 0.6.
The cell line IBW 9 is heterozygous at DR4, w6.
References

Mach BF, et al. DNA sequences coding for the DR-beta-chain locus of the human lymphocyte antigen complex and polypeptides, diagnostic typing processes and products related thereto. US Patent 5,169,941 dated Dec 8 1992

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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