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pRSVZ

規格:

價格:

編號:B230475

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pRSVZ

商品貨號

B230475

Designations

pRSVZ

Depositors

GR Macgregor

培養基

Biosafety Level

Vector Information

Size (kb): 7.8000001907348630

Vector: pRSVZ (plasmid)

Promoters: Promoter RSV LTR

Construction: pRSVcat, pC4AUGbeta-gal

Marker(s):ampR

Construct size (kb): 7.800000190734863

Features: insert detection: lacZ'
marker(s): ampR
promoter: RSV LTR
replicon: pMB1

Applications

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 2.6; XbaI--7.8.

Produces beta-gal under control of RSV LTR, which functions in both Escherichia coli & mammalian tissue culture cells. Co-transfect with other plasmids and determine relative transfection efficiency by normalizing to beta-gal activity.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

MacGregor GR, et al. Histochemical staining of clonal mammalian cell lines expressing E. coli beta galactosidase indicates heterogeneous expression of bacterial gene. Somatic Cell Mol. Genet. 13: 253-265, 1987. PubMed: 2440117

Shipped

freeze-dried

Shipping Information

Distributed: freeze-dried

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