vector for studying DNA-protein interactions tubulin, alpha
Comments
PCR-amplified gene fragments can be ligated in a single step between two opposing T7 promoters. This vector can integrate into a nontranscribed region of the Trypanosoma brucei genome and establish a stable cell line. Tetracycline induction leads to the production of a dsRNA with concomitant degradation of the target mRNA.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Wang Zefeng, et al. Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters. J. Biol. Chem. 275: 40174-40179, 2000. PubMed: 11013266
