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pBKE

規格:

價格:

編號:B230509

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pBKE

商品貨號

B230509

Designations

pBKE

Species

Homo sapiens, human

培養基

Vector

Construct size (kb): 4.03000020980835

Insert

DNA: Synthetic

Insert lengths(kb): 0.0390000008046627

Gene product: immunoglobulin kappa enhancer [IGK*]

Insert Size (kb)

0.039

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--4.1; EcoRI--4.1; PvuII--4.1; HindIII--uncut; EcoRI/BamHI--4.1.

In 70Z/3 cells, kappa enhancer binding activity was absent in unstimulated cells, but was induced in cells incubated with lipopolysaccharide and interleukin-1, forskolin, or BrcAMP.

The sequence of the insert, from the EcoRI to the BamHI sites is: GAATTCGGCA TCTCAACAGA GGGGACTTTC CGAGAGGCGG ATCC. The string of 4 G's begins the 11 bp NF-kappaB-binding site.

References

Shirakawa F, Mizel SB. In vitro activation and nuclear translocation of NF-kappaB catalyzed by cyclin AMP-dependent protein kinase and protein kinase C. Mol. Cell. Biol. 9: 2424-2430, 1989. PubMed: 2548081

Shirakawa F, et al. Interleukin 1 and cyclic AMP induce kappa immunoglobulin light-chain expression via activation of an NF-kappaB-like DNA-binding protein. Mol. Cell. Biol. 9: 959-964, 1989. PubMed: 2542770

B186881：pBKE

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