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pbPKC-gamma19

規(guī)格:

價格:

編號:B230554

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pbPKC-gamma19

商品貨號

B230554

Designations

pbPKC-gamma19

GenBank Number

M13976

培養(yǎng)基

Species

Bos taurus, cow

Depositors

A Ullrich

Vector

Construct size (kb): 4.599999904632568

Insert

DNA: cDNA

Insert lengths(kb): 1.911999940872192

Tissue: brain

Gene product: protein kinase C, gamma [PRKCG]

Insert Size (kb)

1.912

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Insert contains the following restriction sites (with positions according to the published sequence): PstI--678, 1581.

Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.9; PstI--3.7, 0.9; HindIII--4.6.

The probe detects bovine cerebellar mRNA of 3.1 and 2.4 kb and rat brain mRNA of 3.1 kb.

Used to map the human gene. It detects human genomic HindIII fragments of 5.5, 4.6, and 4.3 kb and EcoRI fragments of 7.2, 4.4, and 3.4 kb.

A 170 bp HincII/EcoRI fragment of the insert detects a major genomic bovine PvuII fragment of 10.5 kb and weaker signals at 5.7 and 7.6 kb. In humans this fragment detects a genomic 7.2 kb PvuII fragment.

References

Coussens L, et al. Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways. Science 233: 859-866, 1986. PubMed: 3755548

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