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Tf [TF66G2]
Tf [TF66G2]
規格:
價格:
編號:B230695
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Tf [TF66G2]
商品貨號
B230695
Designations
Tf [TF66G2]
GenBank Number

M12525

培養基
Species
Homo sapiens, human
Depositors
G Lucotte
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 1.0
Gene product: transferrin [TF]
Alleles: B1, B2, A1, A2
Insert Size (kb)
1.0
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.3, 0.8, 0.3; EcoRI--uncut; PvuII--5.6; BamHI--5.6; EcoRI/PvuII--5.6.
The insert contains an internal PstI site (two fragments of 787 and 214 bp).
The insert encodes TF from amino acid 403 to COOH terminus with 166 nucleotides of 3' untranslated region.
Incomplete dominance for the PstI RFLP has been demonstrated in one informative family.
HindIII variant shows amplification of TF sequences.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
Enzyme(s) not detecting polymorphism: EcoRI, PvuII, BamHI, TaqI, MspI.
References

Uzan G, et al. Molecular cloning and sequence analysis of cDNA for human transferrin. Biochem. Biophys. Res. Commun. 119: 273-281, 1984. PubMed: 6322780

le Paslier D, et al. Evolution comparee du gene transferrine chez l'homme et les primates, par hybridation apres transfert avec une sonde specifique. Biochem. Syst. Ecol. 13: 207-212, 1985.

Lavareda de Souza S, Lucotte G. Genetique moleculaire de la transferrine: Localisation chromosomique et variation individuelle du gene. Bio-sciences 4: 139-144, 1985.

Barbara Weiffenbach, personal communication

Gerard Lucotte, personal communication

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