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MKK1a
MKK1a
規(guī)格:
價格:
編號:B230845
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
MKK1a
商品貨號
B230845
Designations
MKK1a
GenBank Number

L05624

培養(yǎng)基
Species
Homo sapiens, human
Depositors
EG Krebs
Applications
in another host, produces protein mitogen-activated protein kinase kinase protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1), MAP kinase kinase
Vector
Construct size (kb): 6.454999923706055
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDNA I/Amp
Intact vector size: 5.000
Type of vector: phagemid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: HindIII BamHI BstXI EcoRI EcoRV BstXI NotI XhoI SphI NsiI
XbaI
Polylinker sites: HindIII BamHI BstXI EcoRI EcoRV BstXI NotI XhoI SphI NsiI
XbaI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: CMV
marker(s): ampR
promoter: CMV, T7, SP6
replicon: polyoma, ColE1, M13
terminator: SV40
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MKK*
Gene name: mitogen-activated protein kinase kinase
Contains complete coding sequence?: U
Tissue: T-lymphocyte
Type of DNA: cDNA
Insert 5' end: BamHI
Insert 3' end: EcoRI
Insert size (kb): 1.455
Cross references: DNA Seq. Acc.: L05624
Insert lengths(kb): 1.455000042915344
Tissue: T-lymphocyte
Gene product: mitogen-activated protein kinase kinase( protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1), MAP kinase kinase) [MKK*]
Target Gene: mitogen-activated protein kinase kinase
Insert Size (kb)
1.455
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRV--5.0, 1.45; BglII--6.5; EcoRI--5.5, 1.0; PvuII--2.8, 1.95, 1.7; HaeII--3.6, 2.2, 0.7.
The insert contains the following restriction sites (bp from the 5' end): AflIII--719; AlwI--486; BanI--860; BglII--1120; BspMII--353; BsrI--1010; BstUI--15; EcoRI--512; HaeII--176; HgiAI--463; HpaI--91;
HphI--1314; PvuII--61; RsaI--405; SspI--1374; TaqI--680.
A probe from nt 1-516 of the insert detects a 2.6 kb mRNA in human thymus, brain, kidney, muscle, liver, intestine, heart, lung, spleen and EGF-stimulated A431 cells.
References

Seger R, et al. Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases. J. Biol. Chem. 267: 25628-25631, 1992. PubMed: 1281467

Edwin G Krebs, personal communication

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