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pCSF25/5

規格:

價格:

編號:B230996

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pCSF25/5

商品貨號

B230996

Designations

pCSF25/5

GenBank Number

M20021

培養基

Species

chloris striate mosaic geminivirus (CSMV)

Depositors

BA Morris

Vector

Construct size (kb): 5.40

Insert

DNA: Genomic

Insert lengths (kb): 2.75

Tissue: DNA from infected grass (Dactyloctenium australe)

Insert Size (kb)

2.75

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--2.75, 2.7; KpnI--4.9, 0.5; PstI--3.15, 1.4, 0.87; SacI--5.4.

The insert contains the following restriction sites (approximate kb from a BamHI site): EcoRI--2.17; KpnI--0.23, 0.69; PstI--0.42, 1.32; SacI--0.94.

Features of the virus occupy the following positions: LIR--(2-233); SIR--(1376-1579); ORF 33.1--(2750-1867); ORF 15.8--(1987-1580); ORF 26.8--(658-1335); ORF 14.5--(234-1375).

Constructed by linearizing the viral ds DNA at the unique BamHI site (nt 351) within the ORF encoding a 14.5 kDa protein.

Classification

Geminivirus

References

Andersen MT, et al. Nucleotide sequence of the Geminivirus chloris striate mosaic virus. Virology 164: 443-449, 1988. PubMed: 3369088

Morris BA, et al. Plant Mol. Biol. 11: 759-803, 1988.

Bret A Morris, personal communication

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