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當前位置: 首頁 > ATCC代理 > pIC-ER-C [pIC 20 ER N11] B231533
pIC-ER-C [pIC 20 ER N11]
pIC-ER-C [pIC 20 ER N11]
規格:
價格:
編號:B231533
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pIC-ER-C [pIC 20 ER N11]
商品貨號
B231533
Designations
pIC-ER-C [pIC 20 ER N11]
GenBank Number

M12674

培養基
Species
Homo sapiens, human
Depositors
H Ahrens
Applications
produces protein estrogen receptor
Vector
Construct size (kb): 3.759999990463257
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pIC20H
Intact vector size: 2.722
Type of vector: plasmid
Vector end: SmaI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI ClaI
EcoRV BglII XhoI NruI
Polylinker sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI ClaI
EcoRV XbaI BglII XhoI SacI NruI HindIII
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1
Cross references: DNA Seq. Acc.: L08912
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ESR
Genomic copy number: unique
Gene name: estrogen receptor
Contains complete coding sequence?: U
Chromosome: 6; Localization: 6 q25.1
Type of DNA: cDNA
Insert 5' end: NaeI
Insert 3' end: EcoRI
Insert size (kb): 1.06
Cross references: DNA Seq. Acc.: M12674
Insert lengths(kb): 1.059999942779541
Gene product: estrogen receptor [ESR]
Alleles: A1, A2
Target Gene: estrogen receptor
Insert Size (kb)
1.060
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9; BglII--3.3, 0.54; HindIII--2.6, 0.88, 0.33; PstI--3.2, 0.64; XbaI--3.4, 0.43; HindIII/SstI--2.7, 0.76, 0.34.
The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.55; HindIII--0.30; NcoI--0.46; PstI--0.60, 0.77; StuI--0.76; XbaI--0.41.
Overproduces the fusion protein in E. coli, the majority of which is found in inclusion bodies.
It binds estrogens and antiestrogens.
Contains coding sequence for amino acids 241-595 including the stop codon.
The recombinant fusion protein contains an additional 18 N-terminal amino acids encoded by the vector polylinker.
References

Ahrens H, et al. Overproduction of full-length and truncated human estrogen receptors in Escherichia coli. Receptor 2: 77-92, 1992. PubMed: 1472947

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