Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
CHLA-01-MED was established from an 8 year old boy with CNS-disseminated medulloblastoma. Cells were isolated from the brain tumor resected at the time of diagnosis and were cultured in neurobasal medium after mechanical disruption. This cell line has been continuously carried in culture for over 18 months, and in neurobasal medium grows as spheres of varying sizes. When placed in medium containing fetal bovine serum the cells attach and spread, but after a period of proliferation they senesce.
A metastatic recurrent medulloblastoma cell line from this patient is available as CHLA-01R-MED (see ATCC CRL-3034)
CHLA-01-MED was established from an 8 year old boy with CNS-disseminated medulloblastoma. Cells were isolated from the brain tumor resected at the time of diagnosis and were cultured in neurobasal medium after mechanical disruption. This cell line has been continuously carried in culture for over 18 months, and in neurobasal medium grows as spheres of varying sizes. When placed in medium containing fetal bovine serum the cells attach and spread, but after a period of proliferation they senesce. The generation of this cell line was made possible with the support of the Michael Hoefflin Foundation for Children's Cancer to Childrens Hospital Los Angeles, with the goal of making pediatric brain tumor lines available to the research community.
A metastatic recurrent medulloblastoma cell line from this patient is available as CHLA-01R-MED (see ATCC CRL-3034)
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
