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pSV2-gpt

規格:

價格:

編號:B231907

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pSV2-gpt

商品貨號

B231907

Designations

pSV2-gpt

Depositors

P Berg

培養基

Biosafety Level

Host

Escherichia coli HB101

Vector Information

Size (kb): 5.50

Vector: pSV2-gpt (plasmid)

Promoters: Promoter SV40

Construction: SV40, pBR322, Escherichia coli

Marker(s): ampR,gpt+

Construct size (kb): 5.5

Features:
marker(s): ampR, gpt+
promoter: SV40
replicon: pMB1
terminator: SV40 early

Applications

Expression vector

Shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): HindIII/BglII--5.2, 0.12; HindIII--5.2; HindIII/EcoRV--4.7, 0.58; PvuII--5.3; PstI--4.3, 0.9; PvuII/PstI--2.8, 1.5, 0.9.

Plasmid containing cells use xanthine as a guanine nucleotide precursor.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37°C

References

Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549

Mulligan RC, Berg P. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc. Natl. Acad. Sci. USA 78: 2072-2076, 1981. PubMed: 7017722

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