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LS129-3C3-E3-1 [3C3]
LS129-3C3-E3-1 [3C3]
規格:
價格:
編號:B231959
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
LS129-3C3-E3-1 [3C3]
商品貨號
B231959
Organism
Mus musculus (B cell); Mus musculus (lymphoma), mouse (B cell); mouse (lymphoma)
Cell Type
hybridoma:lymphoblast B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody binds to the CC chemokine receptor CCR9 (GPR-9-6) and blocks the binding of the thymus-expressed chemokine (TECK) ligand to the receptor.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images
Derivation
Animals were immunized with a peptide consisting of the NH2 terminus of GPR-9-6 having the sequence MADDYGSESTSSMEDYVNFNFTDFYC. Spleen cells were fused with Sp2/0 myeloma cells. The antibody binds to the CC chemokine receptor CCR9 (GPR-9-6) and blocks the binding of the thymus-expressed chemokine (TECK) ligand to the receptor.
Comments
Animals were immunized with a peptide consisting of the NH2 terminus of GPR-9-6 having the sequence MADDYGSESTSSMEDYVNFNFTDFYC. Spleen cells were fused with Sp2/0 myeloma cells. The antibody binds to the CC chemokine receptor CCR9 (GPR-9-6) and blocks the binding of the thymus-expressed chemokine (TECK) ligand to the receptor.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 50 ng/ml mouse recombinant Interleukin-6 (IL-6) and 10% fetal bovine serum
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 3 X 10(4) viable cells/ml.
Interval: Maintain cultures at a cell concentration between 3 X 10(4) and 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
IgG2b
Population Doubling Time
18 hours
Name of Depositor
LeukoSite, Inc.
U.S. Patent Number
References

Zabel BA, et al. Human G protein-coupled receptor GPR-9-6/CC chemokine receptor 9 is selectively expressed on intestinal homing T lymphocytes, mucosal lymphocytes, and thymocytes and is required for thymus-expressed chemokine-mediated chemotaxis.. J. Exp. Med. 190: 1241-1256, 1999. PubMed: 10544196

Andrew DP, et al. Anti-GPR-9-6 antibodies and methods of identifying agents which modulate GPR-9-6 function. US Patent 6,329,159 dated Dec 11 2001

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