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Human Parechovirus 2

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價格:

編號:B232094

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

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產品名稱

Human Parechovirus 2

商品貨號

B232094

Classification

Picornaviridae, Parechovirus

Deposited As

Echovirus 23

培養基

Agent

Human Parechovirus 2

Strain

Williamson

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen 1 mL

Storage Conditions

-70°C or colder

Images

Comments

Derived by adaptation from primary cell culture product ATCC^® VR-53™ to HT-29 cells (ATCC^® HTB-38™). ATCC^® VR-53™ and ATCC^® VR-1820™ have not been compared for sequence or infectivity in primary cell culture and continuous tissue culture.

Effect on Host

CPE, rounding and sloughing

Recommended Host

HT-29 cells (ATCC^® HTB-38™)

Growth Conditions

Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 65-75% confluent, 18-24 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm²). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days at 37°C in a humidified 5% CO₂ atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor

HA Wenner

Source

Rectal swab from child with diarrhea, Ohio, 1956

References

Wenner HA, Lenahan MF. Propagation of group A Coxsackie viruses in tissue cultures. II. Some interactions between virus and mammalian cells. Yale J. Biol. Med. 34: 421-438, 1961. PubMed: 14006144

Westerhuis BM, et al. Growth characteristics of human parechovirus 1 to 6 on different cell lines and cross- neutralization of human parechovirus antibodies: a comparison of the cytopathic effect and real time PCR. Virol. J. 10: 146, 2013. PubMed: 23668373

Wigand R, Sabin AB. Properties of ECHO types 22, 23 and 24 viruses. Arch. Gesamte Virusforsch. 11: 224-247, 1961. PubMed: 13785166

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