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pH15C

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價格:

編號:B232168

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產品名稱

pH15C

商品貨號

B232168

Designations

pH15C

GenBank Number

M57627

培養基

Species

Homo sapiens, human

Depositors

DNAX Research Institute of Molecular and Cellular Biology, Inc., KW Moore, DNAX Research Institute of Molecular and Cellular Biology, Inc.

Applications

in another host, produces protein interleukin 10

Vector

Construct size (kb): 5.5

Insert

DNA: cDNA

Insert lengths(kb): 1.600000023841858

Tissue: T-lymphocyte B21 cell line, activated

Gene product: interleukin 10 [IL10]

Target Gene: interleukin 10

Insert Size (kb)

1.600

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--3.6, 1.9; HindIII--3.7, 1.8; BglII/HindIII--3.7, 1.1, 0.7; PvuII--4.4, 1.1.

The insert contains the following restriction sites (approximate kb from the 5' end): PvuI--0.15, 0.21; HindIII--0.92; EcoRV--1.41.

Contains the complete coding sequence (nt 31-567).

The mobility of the protein produced by COS-7 transfectants is not affected by tunicamycin.

The 3' untranslated region contains an Alu repeat. A 0.7 kb BglII/HindIII fragment from the insert is repeat-free.

The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR).

References

Mosmann TR, et al. Nucleic acids encoding cytokine synthesis inhibitory factor (interleukin-10). US Patent 5,231,012 dated Jul 27 1993

Vieira P, et al. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading fram BCRFI. Proc. Natl. Acad. Sci. USA 88: 1172-1176, 1991. PubMed: 1847510

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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