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pEX2TBP
pEX2TBP
規格:
價格:
編號:B232383
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pEX2TBP
商品貨號
B232383
Designations
pEX2TBP
GenBank Number

M25318

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 2.839999914169312
Insert
DNA: genomic
Insert lengths(kb): 0.136000007390976
Gene product: T cell receptor, gamma, constant region C2 [TCRGC2]
Alleles: B1, D4, D2, A1, A2, B2, D1, D3
Insert Size (kb)
0.136
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 0.14; EcoRI--2.84; HindIII--2.84; PstI--2.84; BamHI--2.84.
Subcloned from lambdaR12. Contains the triplicated exon 2 (ex2T). Cross hybridizes to exon2 of TCRGC1 and to different exons of TCRGC2 (ex2R, ex2).
For the HindIII polymorphism, the 2.7 and 4.1 kb constant bands correspond to TCRGC1 and the 15 kb constant band corresponds to the 5' region of TCRGC2.
In EcoRI digests, the 2.3 kb band corresponds to ex2 of TCRGC1, the 1.4 kb band to TCRGC2 ex2R, and the 1.6 band to TCRGC2 ex2. In PvuII digests, the 9.4 kb band corresponds to the TCRGC1 gene.
References

Buresi C, et al. Exon duplication and triplication in the human T-cell receptor gamma constant region genes and RFLP in French, Lebanese, Tunisian, and Black African populations. Immunogenetics 29: 161-172, 1989. PubMed: 2564370

Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738

Marie-Paule Lefranc, personal communication

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