Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
TT cells continuously produce high levels of calcitonin and CEA.
Immunoreactive calcitonin was found to be produced in cell culture at levels of 3900 pg/million cells and 7700 pg/million cells 24 and 72 hours respectively, after a medium change.
CEA was found to accumulate to greater than 27 ng/million cells over a 72 hours period.
Chromosomal analysis of the cell line and tumors induced in nude mice reveal an aneuploid human karyotype with several marker chromosomes.
The initial characterization studies of the TT cell line were conducted using early passage TT cells cultivated in RPMI 1640 medium supplemented with 15% fetal bovine serum and 1 mM L-glutamine.
It is not known if the neuropeptides reported to be produced by this cell line when it was grown in RPMI 1640 medium are also produced by the cells when they are cultured in Ham's F12K medium.
Chromosomal analysis of the cell line and tumors induced in nude mice reveal an aneuploid human karyotype with several marker chromosomes.
. Advances in thyroid neoplasia, 1981. Rome: Field Educational Italia; 1981.
Behr TM, et al. Improved treatment of medullary thyroid cancer in a nude mouse model by combined radioimmunochemotherapy: doxorubicin potentiates the therapeutic efficacy of radiolabeled antibodies in a radioresistant tumor type. Cancer Res. 57: 5309-5319, 1997. PubMed: 9393755
. Advances in thyroid neoplasia, 1981. Rome: Field Educational Italia; 1981.
Nucleotide (GenBank) : AJ275978 Homo sapiens partial mRNA LAGE-2b for hypothetical protein (LAGE-2 gene).
