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pSPMoIL3 [MOIL3]

規格:

價格:

編號:B233102

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pSPMoIL3 [MOIL3]

商品貨號

B233102

Designations

pSPMoIL3 [MOIL3]

GenBank Number

K01668

培養基

Species

Mus musculus, mouse

Depositors

T Yokota

Vector

Construct size (kb): 4.0

Insert

DNA: genomic

Insert lengths(kb): 0.8999999761581421

Gene product: interleukin 3 (colony-stimulating factor, multiple) [Il3]

Insert Size (kb)

0.900

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

The insert contains internal PstI, HindIII, BamHI BglII, XbaI, and NcoI sites.

Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 0.67, 0.48; SacI--4.0; XbaI--3.4, 0.69; SphI/EcoRI--2.45, 1.5; BglII--4.0; PstI--3.1, 0.94.

The SphI site in the vector is 432 bp from the SalI cloning site. The SphI/EcoRI fragment contains about 500 bp of non-insert DNA (some from pSP64 and some from pcD).

An XhoI fragment from MoIl3 (in a pcD vector) was cloned into the SalI site of pSP64. This fragment contains some of the SV40 promoter and polyadenylation sequences from the pcD vector.

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Yokota T, et al. Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells. Proc. Natl. Acad. Sci. USA 81: 1070-1074, 1984. PubMed: 6322187

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