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AS321
AS321
規格:
價格:
編號:B233167
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
AS321
商品貨號
B233167
Designations
AS321
GenBank Number

M81840

培養基
Species
Homo sapiens, human
Applications
contains sequence DNA Segment, single copy, expressed probes
Vector
Construct size (kb): 4.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS-
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D14S46E
Other gene symbols: NRL; D14S46
Gene name: DNA Segment, single copy, expressed probes
Contains complete coding sequence?: Y
Tissue: retina, adult
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI
Insert 3' end: Modification: HindIII
Insert size (kb): 1.204
Cross references: DNA Seq. Acc.: M81840
Nucleotides 1-1204 of the insert correspond to
nucleotides 24-1227 of M81840.
Insert lengths(kb): 1.203999996185303
Tissue: retina, adult
Gene product: DNA Segment, single copy, expressed probes [NRL]
Target Gene: DNA Segment, single copy, expressed probes
Insert Size (kb)
1.204
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 1.0, 0.2, EcoRI/KpnI--3.0, 1.2; HindIII--4.0, 0.2; SacI--3.0, 1.2; XhoI--3.7, 0.5.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.96; NcoI--0.005, 0.135, 0.39, 0.44; PstI--0.34, 0.40, 0.65, 1.075; PvuII--0.35, 0.49, 0.55, 0.70; SacI--1.07; XhoI--0.78.
In Northern analyses, the insert detects transcripts in retinal tissue of the following species: baboon (1.3 kb); bovine, mouse, and rat (2.0 kb, major); frog (4 kb, 2kb, both faint).
References

Swaroop A, et al. A conserved retina-specific gene encodes a basic motif/leucine zipper domain. Proc. Natl. Acad. Sci. USA 89: 266-270, 1992. PubMed: 1729696

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