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pG36/T1 [B237/L2-T1]

規格:

價格:

編號:B233327

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pG36/T1 [B237/L2-T1]

商品貨號

B233327

Designations

pG36/T1 [B237/L2-T1]

Species

Saccharomyces cerevisiae Meyen ex E.C. Hansen

培養基

Depositors

A Tzagoloff

Vector

Construct size (kb): 21.70000076293945

Insert

DNA: genomic

Insert lengths(kb): 11.0

Gene product: intron processing factor [CBP2]

Insert Size (kb)

11.0

Genotype

MATalpha leu2-3 leu2-112 cbp2-1

Mating Type

alpha

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

The insert contains the following sites separated by (kb) (approximate): BamHI- 2.4- BamHI- 0.33- EcoRI- 0.55- HindIII.

Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--3.9, 3.3, 2.8, 2.3, 1.5, 1.4, 1.0; XhoI--14, 7.8; XbaI--21.7; XbaI/AvaI--7.0, 3.5, 3.2, 2.3, 1.5, 1.4, + others; BamHI--11.0, 4.3, 3.5, 2.3.

The insert includes a 1.89 kb open reading frame encoding a 74 kDa basic protein essential for an early step in splicing the cytochrome b pre-mRNA.

The probe, pG36/T1, has been deposited in Escherichia coli RR1 as ATCC 37662 and in Saccharomyces cerevisiae B237/L2 as ATCC 66318.

Classification

Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae

References

McGraw P, Tzagoloff A. Assembly of the mitochondrial membrane system. Characterization of a yeast nuclear gene involved in the processing of the cytochrome b pre-mRNA. J. Biol. Chem. 258: 9459-9468, 1983. PubMed: 6348045

B195206：pG36/T1 [B237/L2-T1]

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