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pT109luc Plasmid in Escherichia coli

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編號:B233430

品牌:Mingzhoubio

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RNA

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產品名稱

pT109luc Plasmid in Escherichia coli

商品貨號

B233430

Designations

pT109luc Plasmid in Escherichia coli

Depositors

SK Nordeen

培養基

Biosafety Level

Vector Information

Size (kb): 6.09

Vector: pT109luc (plasmid)

Promoters: Promoter HSV TK

Construction: pXP2, herpes simplex virus TK promoter

Marker(s):ampR

Construct size (kb): 6.09

Features: marker(s): ampR
promoter: HSV TK
replicon: pMB1
terminator: none
enhancer: none

Applications

expression vector

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--6.6; HindIII--6.6; SalI--6.6.

There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts.

This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California.

One of a series of luciferase vectors (ATCC 37574-37584) for assaying promoter activity in mammalian cells.

This contains bp -109 to +52 of the herpes simplex tk promoter; both GC + CCAAT are present.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Nordeen SK. Luciferase reporter gene vectors for analysis of promoters and enhancers. BioTechniques 6: 454-458, 1988. PubMed: 2908509

de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727

Steven K Nordeen, personal communication

Shipped

freeze-dried

Shipping Information

Distributed: freeze-dried

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