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CBRM1/10
CBRM1/10
規格:
價格:
編號:B233579
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CBRM1/10
商品貨號
B233579
Organism
Mus musculus, mouse
Tissue
spleen
培養基
Cell Type
hybridoma: lymphoblast B lymphocyte; somatic cell hybridoma
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain
BALB/c
Applications
The monoclonal antibodies produced by these hybridomas will be new insights into how different domains in the integrin alpha and beta subunits may interact.
RefDiamond MS, et al. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J. Cell Biol. 120: 1031-1043, 1993. PubMed: 7679388
Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
Mice (BALB/c) were immunized with purified Mac1 (CD11b/CD18). Spleen cells were fused with P3X63Ag8.653 (ATCC CRL-1580).
Genes Expressed
immunoglobulin; monoclonal antibody against C-terminal epitope of human Mac-1
Cellular Products
immunoglobulin; monoclonal antibody against C-terminal epitope of human Mac-1
Comments
Monoclonal antibodies from selected hybridomas recognized the Mac-1 alpha subunits on the surface of neutrophils. The monoclonal antibodies produced by these hybridomas will be new insights into how different domains in the integrin alpha and beta subunits may interact.
RefLu C, et al. The structure of the beta-propeller domain and C-terminal region of the integrin alphaM subunit. Dependence on beta subunit association and prediction of domains.. J. Biol. Chem. 273: 15138-15147, 1998. PubMed: 9614126
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 104) to 1 X 10(5) viable cells/ ml. Maintain cultures at a cell concentration between 2 X 10(5) and 1 X 10(6) cells/ml.
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
IgG1; Kappa light chain
Name of Depositor
TA Springer
Year of Origin
November 20, 1991
References

Diamond MS, et al. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J. Cell Biol. 120: 1031-1043, 1993. PubMed: 7679388

Lu C, et al. The structure of the beta-propeller domain and C-terminal region of the integrin alphaM subunit. Dependence on beta subunit association and prediction of domains.. J. Biol. Chem. 273: 15138-15147, 1998. PubMed: 9614126

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