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Human adenovirus D
Human adenovirus D
規(guī)格:
價格:
編號:B233612
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Human adenovirus D
商品貨號
B233612
Classification
Adenoviridae, Mastadenovirus
Agent
Human adenovirus D
培養(yǎng)基
Strain
A.A.
Common Name
Human adenovirus 13
Applications
Respiratory research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
?-70°C or colder
Comments
Recognized Reference Strain: Pereira HG, et al.A short description of adenovirus group. Virology 20: 613, 1963.
Agglutinates both rat and rhesus RBC but usually the former to a higher titer than the latter. Contains soluble group-specific complement fixing antigen.
Effect on Host
CPE, rounding and sloughing
Recommended Host
KB cells (ATCC® CCL-17™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 60-90% confluent. Remove medium and inoculate with a small volume (e.g. 1mL per 25 cm squared) of virus diluted with VGM to provide a MOI of about 0.1-1.0. Adsorb 1-2 hours at 37°C in a 5% CO2 atmosphere. End adsorbtion by adding VGM.
Incubation: Incubate infected culture for 1-6 days at 37°C in a 5% CO2 humidified atmosphere until CPE is well advanced through 90% of culture.
Name of Depositor
RJ Huebner, NIH
Chain of Custody
Deposited as Adenovirus type 13
Source
Anal swab from healthy child, Washington, DC, 1955
References

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Rowe WP, et al. Serotype composition of the adenovirus group. Proc. Soc. Exp. Biol. Med. 97: 465-470, 1958. PubMed: 13518306

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