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SM38
SM38
規格:
價格:
編號:B233636
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
SM38
商品貨號
B233636
Designations
SM38
GenBank Number

X14420

培養基
Species
Homo sapiens, human
Depositors
H Kuivaniemi
Vector
Construct size (kb): 7.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: COL3A1
Genomic copy number: unique
Gene name: collagen, type III, alpha 1 (Ehlers-Danlos syndrome type
IV, autosomal dominant)
Contains complete coding sequence?: N
Chromosome: 2; Localization: 2 q31-q32.3
Tissue: skin fibroblasts, neonatal (cell line JIMM-69)
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI linker
Insert 3' end: EcoRI
Insert size (kb): 4.278
Cross references: DNA Seq. Acc.: X14420
Nucleotides 1-4278 of the insert correspond to
nucleotides 25-4303 of X14420.
Insert lengths(kb): 4.277999877929688
Tissue: skin fibroblasts, neonatal (cell line JIMM-69)
Gene product: collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant) [COL3A1]
Insert Size (kb)
4.278
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.4(doublet), 0.9; BglI-- 2.5, 1.4(doublet), 0.9(doublet), 0.52; EcoRI--4.6, 2.9; NcoI--4.7, 3.5; XhoI--3.9, 2.5, 1.1.
Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.25, 1.11; BglI--0.61, 1.94, 2.39, 3.28; EcoRI--4.27; NcoI--0.97, 4.07; PvuII--0.60, 0.84, 2.39, 3.09, 3.25, 4.13; SacI--0.96, 1.29, 2.08, 2.90; XhoI--1.05, 3.57.
The insert contains 78 nt 5' untranslated sequence, and 4200 nt of the coding region. The insert ends at a naturally-occuring EcoRI site at nt 4303 of the sequence record.
References

Tromp G, et al. A single base mutation that substitutes serine for glycine 790 of the alpha1(III) chain of type III procollagen exposes an arginine and causes Ehlers-Danlos syndrome IV. J. Biol. Chem. 264: 1349-1352, 1989. PubMed: 2492273

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