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REP42X
REP42X
規(guī)格:
價(jià)格:
編號(hào):B233798
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
REP42X
商品貨號(hào)
B233798
Designations
REP42X
Depositors
SL Forsburg
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 8.5000000000000000
Vector: REP42X (phagemid)
Promoters: Promoter for expression nmt1* (weaker nmt1)
Construction: pUC119
Marker(s):ampR,ura4+
Construct size (kb): 8.5
Features: marker(s): ampR
marker(s): ura4+
promoter for expression: nmt1* (weaker nmt1)
replicon: ars1
replicon: f1
replicon: pMB1
MCS: Xho...SmaI
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--5.7, 1.7, 1.1; EcoRI--7.2, 1.3; XhoI--8.5.
REP3X (ATCC 87603) and REP4X (ATCC 87604) contain full strength nmt1 promoter whereas REP41X (ATCC 87605) and REP42X (ATCC 87607) with medium strength (nmt1*) and REP81X (ATCC 87606) and REP82X (ATCC 87608) with low strength (nmt1**)
due to mutation in the TATA box by Basi et al. (Gene 123:131-136, 1993).
REP41X (ATCC 87605) and REP42X (ATCC 87607) are different in that REP41X contains the LEU2 marker and REP42X, ura4+.
REP42X was constructed by deleting the ATG in the polylinker of the original REP42 plasmid. An XhoI linker was cloned into the blunted NdeI-BamHI cut REP42 plasmid, deleting SalI site.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516

Susan L Forsburg, personal communication

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component of:ATCC 87685
Shipped
freeze-dried
Shipping Information

?Freeze dried phagemid in E. coli

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