Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).
Incubation: 1 to 5 days at 37°C, a 5% CO2 in air atmosphere is recommended.
Whitbeck JC, et al. Synthesis, processing, and oligomerization of bovine herpesvirus 1 gE and gI membrane proteins. J. Virol. 70: 7878-7884, 1996. PubMed: 8892910
York CJ, et al. Isolation and identification of infectious bovine rhinotracheitis virus in tissue culture. Proc. Soc. Exp. Biol. Med. 94: 740-744, 1957. PubMed: 13431940
