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mMT-1

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價格:

編號:B234160

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

mMT-1

商品貨號

B234160

Designations

mMT-1

GenBank Number

V00835

培養基

Species

Mus musculus, mouse

Depositors

RD Palmiter

Vector

Construct size (kb): 6.300000190734863

Insert

DNA: genomic

Insert lengths(kb): 1.850000023841858

Tissue: embryo

Gene product: metallothionein 1A (functional)(metallothionein 1, metallothionein 1A) [Mt1]

Alleles: b, a

Insert Size (kb)

1.850

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--6.3; BglI/BglII--2.2, 2.0, 1.4, 0.3 (doublet); EcoRI/HindIII--4.4, 1.9; KpnI--6.4; PstI--4.4, 1.9.

The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.1; BstEII--0.4; BglI--0.57; BglII--0.82; PstI--1.1.

Please note that the promoter is the subject of issued U.S. Patent No. 4,579,821.

Corresponds to sequences from -750 to +1100 where +1 is the transcription start point.

The region between the BglI site (-185 or nt 116 of the published sequence) and the BglII site (+65 or nt 369 of the published sequence) contains the entire metal-responsive promoter.

The vector is a high copy number derivative of pBR322 with a XhoI linker at about 3000 on the standard map).

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Durnam DM, et al. Isolation and characterization of the mouse metallothionein-I gene. Proc. Natl. Acad. Sci. USA 77: 6511-6515, 1980. PubMed: 6935664

Glanville N, et al. Structure of mouse metallothionein-I gene and its mRNA. Nature 292: 267-269, 1981. PubMed: 7254320

Palmiter RD, Brinster RL. Control of DNA sequence transcription. US Patent 4,579,821 dated Apr 1 1986

Scherer G, et al. Gene mapping on mouse chromosome 8 by interspecific crosses: new data on a linkage group conserved on human chromosome 16q. Genomics 5: 275-282, 1989. PubMed: 2571576

Fletcher C, et al. A multilocus linkage map of mouse chromosome 8. Genomics 9: 737-741, 1991. PubMed: 2037298

Richard D Palmiter, personal communication

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