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pPW121

規(guī)格:

價(jià)格:

編號(hào):B234314

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pPW121

商品貨號(hào)

B234314

Designations

pPW121

Depositors

F Puta

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 4.3860001564025880

Vector: pPW121 (plasmid)

Promoters: Promoter lambda PR

Construction: pUN121

Marker(s):ampR,tetR

Construct size (kb): 4.386000156402588

Features: marker(s): ampR
marker(s): tetR
operator: lac
promoter: lambda PR
replicon: pMB1
repressor gene: cI

Applications

vector permitting positive selection for inserts

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; HindIII--4.3; BamHI--4.3.

Postive selection cloning vector which confers tetR (recommended concentration 10 ug/ml) only when an insert has been successfully cloned into the cI repressor gene.

Plasmid was derived from pUN121 by introduction of a BglII site into the cI gene. Plasmid pUN121 was derived from pTR262 by addition of EcoRI and SmaI/XmaI sites in the cI gene.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Puta F, Wambutt R. Construction of a new Escherichia coli-Saccharomyces cerevisiae shuttle plasmid cloning vector allowing positive selection for cloned fragments. Folia Microbiol. 37: 193-198, 1992. PubMed: 1505881

Nilsson B, et al. An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis. Nucleic Acids Res. 11: 8019-8030, 1983. PubMed: 6316281

Frantisek Puta, personal communication

Shipped

freeze-dried

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