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pJY4313 [JY4313]
pJY4313 [JY4313]
規格:
價格:
編號:B234487
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pJY4313 [JY4313]
商品貨號
B234487
Designations
pJY4313 [JY4313]
GenBank Number

M74122

培養基
Species
Streptococcus pneumoniae (Klein) Chester
Depositors
Univ. Alabama, DE Briles, JL Yother, Univ. Alabama
Applications
produces protein pneumococcal surface protein A, 84 kDa
Vector
Construct size (kb): 4.599999904632568
Insert
DNA: genomic
Insert lengths(kb): 2.085999965667725
Gene product: pneumococcal surface protein A, 84 kDa [pspA]
Target Gene: pneumococcal surface protein A, 84 kDa
Insert Size (kb)
2.086
Media
ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): HindIII/KpnI--2.7, 2.1; PvuII--2.4, 1.3, 0.96; SacI--3.5; 1.3.
Insert contains the following restriction sites (approximate kb from the 5' end): PvuII--1.13, 1.19; SacI--0.79.
The plasmid affects the growth of transformed bacteria and viability in stationary phase. Growth at 30C reduces product degradation.
The following primers can be used to amplify a 0.678 kb pspA product from the insert or genomic DNA (nt 1312-1990 of the published sequence): LSM1 5'-CCGGATCCAGCTCCTGCACCAAAAC-3'; LSM2 5'-GCGCTGCGACGGCTTAAACCCATTCACCATTGG-3'.
References

Briles DE, et al. Structural gene of pneumococcal protein. US Patent 5,476,929 dated Dec 19 1995

Yother J, et al. Truncated forms of PspA that are secreted from Streptococcus pneumoniae and their use in functional studies and cloning of the pspA gene. J. Bacteriol. 174: 610-618, 1992. PubMed: 1729250

David E Briles, personal communication

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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