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SYN A4-1
SYN A4-1
規(guī)格:
價(jià)格:
編號:B234927
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SYN A4-1
商品貨號
B234927
Designations
SYN A4-1
Species
Bacillus thuringiensis subsp. israelensis de Barjac
培養(yǎng)基
Depositors
Syntro Corporation
Vector
Construct size (kb): 41.0
Insert
DNA: genomic
Insert lengths(kb): 11.0
Gene product: crystal protein (delta endotoxin)
Insert Size (kb)
11.0
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried bacteria-free lysate
Comments
The insert contains the following restriction sites (in approximate kb from the end upstream of the gene): EcoRI--2.5, HpaI--4.0; PstI--6.7; BglII--7.3; HindIII--8.0; EcoRI--10.0.
Restriction digests of the clone give the following sizes (kb): EcoRI--23.0, 9.0, 3.5, 3.0, 2.3; BamHI-->23.0, 11.0; HindIII-->23.0, 9.0, 4.8, 3.1.
The central EcoRI fragment of this construct, and a HpaI/EcoRI fragment derived from that, contain the sequences encoding the complete polypeptide.
The insert contains 2 open reading frames. Sequences up to a BalI site at 5.5 kb from the upstream region and after a StuI site in the second reading frame can be deleted without destroying the toxicity of the product to mosquito larvae.
References

Walfield AM, Pollock TJ. Methods and compositions for expression of BTI endotoxin. US Patent 4,652,628 dated Mar 24 1987

Thorne L, et al. Structural similarity between the Lepidoptera- and Diptera-specific insecticidal endotoxin genes of thuringiensis subsp. J. Bacteriol. 166: 801-811, 1986. PubMed: 3011746

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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