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HRD1.1
HRD1.1
規(guī)格:
價(jià)格:
編號(hào):B235231
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
HRD1.1
商品貨號(hào)
B235231
Designations
HRD1.1
GenBank Number

L03411

培養(yǎng)基
Species
Homo sapiens, human
Depositors
JE Volanakis
Vector
Construct size (kb): 4.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7, T3
replicon: pMB1, f1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RDBP
Other gene symbols: D6S45
Genomic copy number: unique
Gene name: RD protein
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 p21.3
Tissue: acute T cell leukemia, Jurkat cell line
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.3
Cross references: DNA Seq. Acc.: L03411
Insert lengths(kb): 1.299999952316284
Tissue: acute T cell leukemia, Jurkat cell line
Gene product: RD protein( major histocompatibility complex gene RD, major histocompatibility complex, class III, RD) [RDBP]
Insert Size (kb)
1.300
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglI--2.4, 1.4, 0.64; EcoRI--2.9, 1.4; HindIII--2.9, 0.68 (doublet); PstI--2.9, 1.2, 0.24; PvuII--2.5, 1.3, 0.52.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.13; PvuII--0.29; HindIII--0.58, 1.30; BglI--0.31; SacI--1.03.
Contains the complete coding sequence (nt 87-1229).
References

Cheng J, et al. cDNA cloning and characterization of the protein encoded by RD, a gene located in the class III region of the human major histocompatibility complex. Biochem. J. 294: 589-593, 1993. PubMed: 8373374

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