国产精品操,三级网站在线播放,黄av在线

YCp50-poly

規(guī)格:

價格:

編號:B235312

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產(chǎn)品名稱

YCp50-poly

商品貨號

B235312

Designations

YCp50-poly

Depositors

DJ Stillman

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 7.0560002326965330

Vector: YCp50-poly (plasmid)

Construction: YCp50, pUC19 polylinker

Marker(s):URA3,ampR

Construct size (kb): 7.056000232696533

Features: marker(s): URA3
marker(s): ampR
replicon: ARS1
replicon: pMB1
MCS: EcoRI...HindIII
centromere: CEN4

Applications

YC-type (centromeric) shuttle vector

vector for constructing genomic libraries

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; SmaI--5.1, 2.2; PstI--3.3, 1.8, 1.1, 0.9; PvuI--7.2.

YC-type shuttle vector.

YCp50-poly was constructed in two steps: (1) YCp50 (ATCC 37419) was cleaved with XhoI and Asp718, blunted and ligated. (2) This construct was then cleaved with EcoRI and SalI, and the EcoRI-PvuII fragment of pUC19 was inserted.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Rose MD, et al. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237-243, 1987. PubMed: 3327750

Wang H, Stillman DJ. Transcriptional repression in Saccharomyces cerevisiae by a SIN3-lexA fusion protein. Mol. Cell. Biol. 13: 1805-1814, 1993. PubMed: 8441414