Construction: lambda pR promoter and cI857 repressor cloned into pCRII, ligated to pSC101/par and tetR from pLG339
Marker(s):tetR
Construct size (kb): 5.0479998588562
Features: marker(s): tetR other: par locus promoter: lambda PR replicon: pSC101 repressor gene: cI857
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): XbaI - 5.0; XhoI - 5.0; PstI - 5.0.
Low copy number expression vector for overexpression of cloned bacterial* genes in E. coli from the lambda pR promoter, induced by temperature or pH shift, (the latter according to literature only).
Media
ATCC® Medium 6025: LB Medium with 12 mcg/ml tetracycline
Growth Conditions
Temperature: 30.0°C
References
Gotting C, et al. Versitile low-copy number plasmids for temperature-inducible overexpression of bacterial genes in Escherichia coli. BioTechniques 24: 362-366, 1998. PubMed: 9526640
