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pcD-2A-E3 [pcD-IL-BSF1/TCGFII/MCGFII]

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編號:B236042

品牌:Mingzhoubio

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DNA

RNA

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產品名稱

pcD-2A-E3 [pcD-IL-BSF1/TCGFII/MCGFII]

商品貨號

B236042

Designations

pcD-2A-E3 [pcD-IL-BSF1/TCGFII/MCGFII]

GenBank Number

M13238

培養基

Species

Mus musculus, mouse

Depositors

DNAX Research Institute, SC Macevicz, DNAX Research Institute

Applications

in suitable host, produces protein B-cell stimulatory factor 1

Vector

Construct size (kb): 3.6

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDV1
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI KpnI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 late
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: mouse
Gene symbol: bcsf1*
Gene name: B-cell stimulatory factor 1
Contains complete coding sequence?: Y
Tissue: T-cell cell line Cl.Ly1+2-1/9 (ATCC CRL-8179)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.6
Cross references: DNA Seq. Acc.: M13238
Nucleotides 1-585 of the insert correspond to
nucleotides 1-585 of M13238.

Insert lengths(kb): 0.60

Tissue: T-cell cell line Cl.Ly1+2-1/9 (ATCC CRL-8179)

Gene product: B-cell stimulatory factor 1 [bcsf1*]

Target Gene: B-cell stimulatory factor 1

Insert Size (kb)

0.600

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Nucleotides 1-585 of the insert correspond to nucleotides 1-585 of M13238.

The insert contains the following unique restriction sites: SacI; AhaIII; PstI(although there are two sites in the vector); ClaI; XmnI; ScaI.

Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 0.8; EcoRI--3.6; HindIII--3.6.

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Lee F, et al. Isolation and characterization of a mouse interleukin cDNa clone that expresses B-cell stimulatory factor 1 activities and T-cell and mast-cell-stimulating activities. Proc. Natl. Acad. Sci. USA 83: 2061-2065, 1986. PubMed: 3083412

Lee F, et al. cDNA clones coding for human protein exxhibiting a broad cellular activity spectrum (human interleukin-4). US Patent 5,552,304 dated Sep 3 1996

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

B177059：p2A-E3 [pCD-2A-E3]

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