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pHisGal

規(guī)格:

價格:

編號:B236333

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pHisGal

商品貨號

B236333

Designations

pHisGal

Depositors

ML Schmitz

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 3.9119999408721920

Vector: pHisGal (phagemid)

Promoters: Promoter for expression lac

Construction: pQE9, Gal4 DNA-binding domain

Marker(s):ampR

Construct size (kb): 3.911999940872192

Features: marker(s): ampR
operator: Gal4 DNA-binding domain (aa 1-147)
promoter for expression: lac
replicon: pMB1
MCS: SmaI...HindIII
restriction site: BamHI
coding sequence: promoterless cat gene
epitope tag: hexahistidine sequence (6-His)

Applications

expression vector

vector permitting construction of fusion proteins

Comments

Restriction digests of the clone give the following sizes (kb): HindIII--4.0; PvuI--4.0; XbaI--4.0.

Plasmid contains the following restriction sites (approximate kb from the BamHI site): XbaI--1.51; PvuI--3.15.

Expression vector for production of Gal4 fusion proteins, allowing protein purification by affinity chromatography through a nickel nitrilotriacetic acid (Ni-NTA) column.

For expression of cloned inserts, the medium should contain 10 uM zinc chloride and expression can be induced using IPTG.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Schmitz ML, Baeuerle PA. A vector, pHisGal, allowing bacterial production of proteins fused to a hexahistidine-tagged Gal4 DNA-binding domain. BioTechniques 17: 714-718, 1994. PubMed: 7833034

Shipped

freeze-dried

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