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YIp358R
YIp358R
規(guī)格:
價格:
編號:B236580
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
YIp358R
商品貨號
B236580
Designations
YIp358R
Depositors
CJ Lusty
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 7.0999999046325680
Vector: YIp358R (plasmid)
Promoters: Promoter none
Construction: YEp358R (ATCC 37739), YIp352
Marker(s):URA3,ampR
Construct size (kb): 7.099999904632568
Features: insert detection: lacZ
marker(s): ampR, URA3
promoter: none
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
promoter-cloning vector
shuttle vector
Comments
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-2; SphI-3; PstI-3; SalI-2; XbaI-2; BamHI-2; SmaI-3; KpnI-3; SacI-3; EcoRI-2. The SacI site is not unique.
Cloning into the HindIII, SphI, PstI, SalI, or XbaI sites leads to a TAG stop codon within the downstream XbaI site of the multiple cloning region.
Restriction digests of the clone give the following sizes (kb): EcoRI-7.1; SalI-7.1; PstI-7.1; HindIII-7.1.
One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37755 - 37757) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'AA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CCC GGG TAC CGA GCT CGA ATT CCC AGC TTC GAT CCC3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915

Shipped
freeze-dried
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