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NU-DUL-1

規(guī)格:

價(jià)格:

編號(hào):B236760

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

NU-DUL-1

商品貨號(hào)

B236760

Organism

Homo sapiens, human

Tissue

peritonial effusion; derived from metastatic site: malignant cerebrospinal fluid

培養(yǎng)基

Cell Type

lymphocyte

Product Format

frozen

Morphology

early B-cell

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

undifferentiated lymphoma, non-Burkitt's type

Age

43 years

Gender

male

Ethnicity

Caucasian

Applications

The Nu-DUL-1 cell line is a valuable resource for studying the biological behavior of undifferentiated lymphoma cells.
The Nu-DUL-1 cell line will aid molecular and cellular studies designed to identify the biological significance of genomic rearrangements.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

44, X (hypodiploid), +der(4)t(4;?)(q31;?). -6, -17, -18, -21, +2mar,der(1)t(1;?)(q32;?), del(4)(q25q31), del(5)(p12), del(6)(q21), del(8)(q24), der(11)t(11;?)(q21;?), der(12)t(12;?)(p13;?), der(14)t(14;?)(q13;), ter rea(19;21)(q13;q22)

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Clinical Data

43 years
Caucasian
Male

Antigen Expression

LN-1+
LN-2+
LN-3+(anti-HLA-Dr)
BA-1+ (CD24)
CALLA (J5)(common ALL antigen; CD10)+
BA-2 - (CD9)
OKM1 - (myeloid marker)
Leu-7 - (natural killer T cell marker)
OKT-3 -, OKT-4 -, OKT-6 -, OKT-8 -, OKT-11 - (T cell markers)
BM-1 -, BM-2 -, BM-3 - ,BM-4 -, BM-5 - (myeloid markers)

EBNA - (Epstein-Barr Nuclear Antigen)

Genes Expressed

sIg -

Comments

The depositor states that this cell line is negative for the Epstein-Barr Virus (EBV).
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.

The NU-DUL-1 cell line represents a very unusual undifferentiated lymphoma.
The NU-DUL-1 cell line has a chromosome 14 translocation, but not from chromosome 11 or 18 and may represent a new variant.
This cell line is hypodiploid with the presumed loss of a Y chromosome.
NU-DUL-1 cells express markers of early B-cell differentiation.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Cultures can be maintained by the addition of fresh medium. An inoculum of 2.0 x 10⁵to 3.0 x 10⁵cells/mL is recommended. Subculture when the cell concentration is between 8.0 x 10⁵ and 1.0 x 10⁶ cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:5 is recommended.
Medium renewal: every 2 to 3 days

Cryopreservation

Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

STR Profile

D5S818: 12, 14
D13S317: 8, 13
D7S820: 11, 13
D16S539: 11, 13
vWA: 16, 17
THO1: 9, 9.3
Amelogenin: X
TPOX: 8
CSF1PO: 11, 12

Name of Depositor

A Epstein

Year of Origin

1985

References

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

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