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當(dāng)前位置: 首頁 > ATCC代理 > pHPT31 B236910
pHPT31
pHPT31
規(guī)格:
價格:
編號:B236910
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pHPT31
商品貨號
B236910
Designations
pHPT31
Species
Homo sapiens, human
培養(yǎng)基
Insert
DNA: cDNA
Insert lengths(kb): 0.949999988079071
Tissue: central nervous system
Gene product: hypoxanthine phosphoribosyltransferase [HPRT]
Alleles: A1, A2, A3
Insert Size (kb)
0.950
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.5, 1.0; SalI--2.25, 0.95; PstI--2.5, 0.93; EcoRI--2.4, 0.99.
The clone was constructed by excising the PstI fragment encoding the full-length cDNA from pHPT30 and inserting the fragment into pUC7. The insert includes about 100 bp 5' untranslated and some 3' untranslated sequence, but not the AATAAA sequence.
The insert detects the same BamHI polymorphism detected by the probe, pDSK1.
Enzyme(s) not detecting polymorphism: EcoRI, HindIII, MspI, KpnI, TaqI.
References

Jolly DJ, et al. Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyltransferase. Proc. Natl. Acad. Sci. USA 80: 477-481, 1983. PubMed: 6300847

Brennand J, et al. Expression of human and Chinese hamster hypoxanthine-guanine phosphoribosyltransferase cDNA recombinants in cultured Lesch-Nyhan and Chinese hamster fibroblasts. J. Biol. Chem. 258: 9593-9596, 1983. PubMed: 6688416

Melton DW, et al. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene. Proc. Natl. Acad. Sci. USA 81: 2147-2151, 1984. PubMed: 6326107

C. Thomas Caskey, personal communication

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