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<em>Saccharomyces cerevisiae</em> Meyen ex E.C. Hansen
<em>Saccharomyces cerevisiae</em> Meyen ex E.C. Hansen
規(guī)格:
價格:
編號:B237255
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Saccharomyces cerevisiae Meyen ex E.C. Hansen
商品貨號
B237255
Deposited As
Saccharomyces cerevisiae Hansen, teleomorph
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
培養(yǎng)基
Strain Designations
MW3317-21A pms1-delta
Alternate State
Candida robusta Diddens et Lodder
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen
Type Strain
no
Genotype
MATalpha pms1-delta trp1 ade8-deltaKpn ura3-52 hom3-10 met13 met4 ade2 his3-Kpn RefWilliamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926, RefKramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974, (Isogenic with MW3317-21A (ATCC 208350) except for the pms locus.)
Preceptrol&reg;
no
Mating Type
alpha
Ploidy
haploid
Comments
Meiosis-sporulation: postmeiotic segregation increase (CL2)
Medium
ATCC® Medium 1069: YPAD medium
Growth Conditions
Temperature: 25.0°C
Atmosphere: Typical aerobic
Protocol: The "quick" method for checking pms1 is to print on canavanine media: papillation (mutability) will be increased. The same result is obtained by printing on met- media and treating with UV; increased papillation will occur due to enhanced mutability at the hom3-10 locus in the presence of the pms1 mutation.
Subcultivation
Protocol: The "quick" method for checking pms1 is to print on canavanine media: papillation (mutability) will be increased. The same result is obtained by printing on met- media and treating with UV; increased papillation will occur due to enhanced mutability at the hom3-10 locus in the presence of the pms1 mutation.
Name of Depositor
YGSC
Special Collection
Yeast Genetic Stock Center
Chain of Custody
ATCC <<--YGSC<<--W. Kramer and K. Lusnak
References

Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926

Kramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974

Isogenic with MW3317-21A (ATCC 208350) except for the pms locus.

Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926

Kramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974

Isogenic with MW3317-21A (ATCC 208350) except for the pms locus.

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